16S rRNA基因测序引物
| Region | Primer name | Sequence (5′–3′) | Reference |
|---|---|---|---|
| V1–V2 | 27F | AGAGTTTGATYMTGGCTCAG | 44 |
| 338R | TGCTGCCTCCCGTAGGAGT | 44 | |
| V1–V3 | 27F | AGAGTTTGATYMTGGCTCAG | 3 |
| 534R | ATTACCGCGGCTGCTGG | 3 | |
| V1–V9 | 27F | AGAGTTTGATCMTGGCTCAG | 1 |
| 1492R | TACGGYTACCTTGTTAYGACTT | 1 | |
| V3 | 341F | CCTACGGGAGGCAGCAG | 12 |
| 518R | ATTACCGCGGCTGCTGG | 12 | |
| V3–V4 | 341F | CCTACGGGNGGCWGCAG | 36 |
| 785R | GACTACNNGGGTATCTAATCC | 36 | |
| V4 | 515F | GTGYCAGCMGCCGCGGTAA | 45 |
| 806R | GGACTACNVGGGTWTCTAAT | 46 | |
| V4–V5 | 515F | GTGYCAGCMGCCGCGGTAA | 45 |
| 926R | CCGTCAATTCMTTTRAGT | 45 | |
| V5–V7 | 799F | AACMGGATTAGATACCCKG | 47 |
| 1193R | ACGTCATCCCCACCTTCC | 47 | |
| V6 | 967F | CAACGCGAAGAACCTTACC | 12 |
| 1046R | CGACAGCCATGCANCACCT | 12 | |
| V6–V8 | 926F | AAACTYAAAKGAATTGACGG | 48 |
| 1392R | ACGGGCGGTGTGTRC | 48 | |
| V7–V9 | 1115F | CAACGAGCGCAACCCT | 2 |
| 1492R | TACGGYTACCTTGTTACGACTT | 2 |
V3-V4引物也用338F (5′-ACTCCTACGGGAGGCAGCA-3′) and 806R (5′- GGACTACHVGGGTWTCTAAT-3′)，参考：Nutrient supply controls the linkage between species abundance and ecological interactions in marine bacterial communities，派森诺测序使用的也是此引物对（标准细菌16S V3V4(a)）
来源：https://journals.asm.org/doi/10.1128/aem.02108-22#T2